超快拉曼成像 FIRE Edge 300

FIRE Edge 300

超快拉曼成像升级

1-minute chemical imaging
Pixels: 200 x 200
Total time: 55 s
(20 s exposure time, 35 s extra time)
Dwell time: 0.5 ms
(25 μs x 20 avg)
超快拉曼成像升级

FIRE imaging of intact daf-2 C. elegans

FIRE imaging of intact daf-2 C. elegans

FIRE spectra obtained in b

FIRE spectra obtained in b

配置

Total time: 55 s
Exposure time: 20 s
Extra time: 20 s
Data processing: 8 s
Image flash: 7 s

Photon-Counting Raman at MHz Spectral Rate

Dwell time: 0.5 ms/pixel
15x15=225 pixels
CCD can not do it

Chemical imaging of D7-glucose in C. elegans

a FIRE spectrum of D7-glucose

FIRE spectrum of D7-glucose

b Detection limit of D7-glucose

Detection limit of D7-glucose

c FIRE Spectra obtained in d

FIRE Spectra obtained in d

光谱参数

SPECTRAL PARAMETERS

类型 参数
激发波长 532 ± 0.5nm
脉冲宽度 100 ps
重复频率 1 MHz
光谱范围 60-4000 cm⁻¹
光栅刻线 1800 l/mm
最大光谱采集速度 1 000 000 条/秒
最小积分时间 1 μs
探测器 单光子计数

成像参数

IMAGING PARAMETERS

类型 参数
视场 300 x 300 μm²
空间分辨率 300 nm(60X物镜, NA 1.2)
像素 200 x 200
积分时间 25 μs-10 s
X-Y电动行程 45 x 75 mm²
物镜电动行程 25 mm
电动模块切换 8通道可选
重复定位精度 ±1 μm
双轴激光扫描 0.1 μm/step

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